Selected Publications
Bevilacqua C, Hambura S, Bouvet P, La Cavera III S, Elsayad Kc, Prevedel Rc. "A standardized file format and open-source analysis framework for Brillouin microscopy data." (preprint)
Czibula C, Simoes MG, Ulz MH, Ravanello BB, Elsayad K, Hirn U, Koski KJ. "The potential of Brillouin Spectroscopy for investigating the mechanical properties of hydrogels during dehydration." (preprint)
Kothmayer M, Laffer S, Widmayer P, Ebner EE, Ugarak F, Martin D, Geyer SH, Elsayad K, Weninger WJ, Lagger S, Weipoltshammer K, Pusch O, Schöfer C. "Aging Reduces Intestinal Stem Cell Activity in Killifish and Intermittent Fasting Reverses Intestinal Gene Expression Patterns." Aging Cell e70229 (2025).
Bouvet P, Bevilacqua C, Ambekar Y, Antonacci G, Au J, Caponi S, Chagnon-Lessard S, Czarske J, Dehoux T, Fioretto D, Fu Y, Guck J, Hamann T, Heinemann D, Jähnke T, Jean-Ruel H, Kabakova I, Koski K, Koukourakis N, Krause D, La Cavera III S, Landes T, Li J, Margueritat J, Mattarelli M, Monaghan M, Overby DR, Perez-Cota F, Pontecorvo E, Prevedel R, Ruocco G, Sandercock J, Scarcelli G, Scarponi F, Testi C, Török P, Vovard L, Weninger WJ, Yakovlev V, Yun S-H, Zhang J, Palombo Fc, Bilenca Ac, Elsayad Kc. "Consensus Statement on Brillouin Light Scattering Microscopy of Biological Materials." Nature Photonics 19:681 (2025).
DOI:10.1038/s41566-025-01681-6
Elsayad K. Brillouin Light Scattering Anisotropy Microscopy. Proc. SPIE 13320, Biomedical Light Scattering XV, 1332005 (2025).
Lindner FP, Strasser N, Schultze M, Wieser S, Slugovc C, Elsayad K, Koski KJ, Zojer E, Czibula C. "Combining Brillouin spectroscopy and machine learned interatomic potentials to probe mechanical properties of metal organic frameworks." J. Phys. Chem. Lett. 16(5):1213 (2025).
DOI:10.1021/acs.jpclett.4c03070
Yao Y, Yao J, Xiong S, Sun Y, Lai L, He C, Jiang S, Elsayad K, Peng H, Wu A, Yang F. "Borrow Strength to Exert: Low-Crystallinity Prussian Blue for Reduction Overload Enhanced Photothermal Therapy." Small 2406145 (2025).
Mosca E, Federa A , Pirker C, Schosserer M, Liendl L , Eckhard M, Sombke A, Dömötör O, Kirchhofer D, Timelthaler G, Baier D, Gurschka P, Gabler L, Reithofer M, Min Chin J, Elsayad K, Englinger B, Tahir A, Kowol CR, Berger W. "The tyrosine kinase inhibitor Nintedanib induces lysosomal dysfunctionality: Role of protonation-dependent crystallization processes." Chem. Biol. Interact. 403:111243 (2024).
Illibauer J, Clodi-Seitz T, Zoufaly A, Aberle J, Weninger WJ, Födinger M, Elsayad Kc. "Diagnostic potential of blood plasma longitudinal viscosity measured using Brillouin light scattering." Proc. Nat. Acad. Sci. USA 121(33):e2323016121 (2024).
Czibula C, Ulz MH, Wagner A, Elsayad K, Hirn U, Koski KJ. "The elastic stiffness tensor of cellulosic viscose fibers measured with Brillouin spectroscopy." J. Phys. Photonics 6(3):035012 (2024).
Keshmiri H, Cikes D, Samalova M, Schindler L, Appel L-M, Urbanek M, Yudushkin I, Slade D, Weninger WJ, Peaucelle A, Penninger J, Elsayad Kc. "Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells." Nature Photonics 18:276 (2024).
DOI:10.1038/s41566-023-01368-w
Windberger U, Sparer A, Elsayad K. "The role of plasma in the yield stress of blood." Clin. Hemorheol. Microcirc. 84(4):369-383 (2023).
Pruidze P, Chayleva E, Weninger WJ, Elsayad Kc. "Brillouin Scattering Spectroscopy for studying human anatomy: Towards in situ mechanical characterization of soft tissue." J. Eur. Opt. Soc. – Rapid Publication 19:31 (2023).
Cikes Dc, Elsayad Kc, Sezgin E, Koitai E, Ferenc T, Orthofer M, Yarwood R, Heinz L, Sedlyarov V, Miranda ND, Taylor A, Grapentine S, al- Murshedi F, Abott A, Weidinger A, Kutchukian C, Sanchez C, Cronin SJF, Novatchkova M, Kavirayani A, Schuetz T, Haubner B, Haas L, Hagelkruys A, Jackowski1 S, Kozlov A, Jacquemond V, Knauf C, Superti-Furga G, Rullman E, Gustafsson T, McDermot J, Lowe M, Radak Z, Chamberlain JS, Bakovic M, Banka S, Penninger JMc. "Loss of PCYT2 causes lipid deficiency-induced muscle degeneration by distorting lipid bilayer architecture." Nature Metabolism, 5, 495–515 (2023).
DOI:10.1038/s42255-023-00791-1
Samalova, M, Melnikava A, Elsayad K, Peaucelle A, Gahurova E, Gumulec J, Spyroglou I, Zemlyanskaya EV, Ubogoeva EV, Hejatko J. "Expansin-controlled cell wall stiffness regulates root growth in Arabidopsis." Plant Physiology, kiad228 (2023).
Antonacci G, Elsayad K, Polli D. "On-chip notch filter on a silicon nitride ring resonator for Brillouin spectroscopy." ACS Photonics 9, 772–777 (2022).
DOI:10.1021/acsphotonics.2c00005
Keshmiri, H, Armin, F, Elsayad, K, Schreiber F, Moreno, M. "Leaky and waveguide modes in biperiodic holograms." Sci. Rep. 11, 10991 (2021).
DOI:10.1038/s41598-021-89971-1
Elsayad K, Brillouin Microscopy in “Imaging Modalities for Biological and Preclinical Research: A Compendium, Volume 1”, Part I: Ex vivo biological imaging (Ed. Walter A, Mannheim J, Caruana CJ), I.9.c-1 – I.9.c-16 (2021) [ISBN 978-0-7503-3057-2].
Kaufmann T, Herbert S, Hackl B, Besold JM, Schramek C, Gotzmann J, Elsayad K, Slade D. "Direct measurement of protein-protein interactions by FLIM-FRET at UV laser-induced DNA damage sites in living cells." Nuc. Acid Res. gkaa859 (2020).
van der Wal E, Cikes D, Olie CS, Maton L, de Greef JC, Lin I-H, Chen Y-F, Elsayad K, Penninger JM, Kessler BK. "Cytoskeleton disorganization underlies aberrant muscle cell function in PABPN1-mediated muscle atrophy." Sci. Rep.10:17621 (2020).
DOI:10.1038/s41598-020-74676-8
Antonacci G, Beck T, Bilenca A, Czarske J, Elsayad Kc, Guck J, Kim K, Krug B, Palombo F, Prevedel Rc, Scarcelli G. "Recent progress and current opinions in Brillouin Microscopy for life science application." Biophys. Rev. 12(3) (2020).
DOI:10.1007/s12551-020-00701-9
Elsayad K, Urstöger G, Czibula, C, Teichert C, Jaromir G, Balvan J, Pohlt M, Hirn U. "Mechanical Properties of cellulose fibers measured by Brillouin spectroscopy." Cellulose (2020).
DOI:10.1007/s10570-020-03075-z
Elsayad K. "Mapping the phonon dispersion in biological matter using angle-resolved Brillouin light scattering microspectroscopy." Proc. SPIE 11242, 112420F (2020).
Elsayad Kc, Palombo F, Dehoux T, and Fioretto D. "Brillouin Light Scattering Microspectroscopy for Biomedical Research and Applications: introduction to feature issue." Biomed. Opt. Exp. 10(5):2670-2673 (2019).
Elsayad K. "A new angle on mapping the refractive index." APS Physics 12, 27 (2019).
https://physics.aps.org/articles/v12/27
Elsayad, K. "Optical imaging spectroscopy for plant research: More than a colorful picture." Current Opinions in Plant Biology 52:77-85 (2019).
Elsayad K, Polakova S, Gregan J. "Probing Mechanical Properties in Biology using Brillouin Microspectroscopy." Trends in Cell Biology S0962-8924(19):30075-3 (2019).
Elsayad K. "Spectral Phasor Analysis for Brillouin Microspectroscopy." Frontiers in Physics 7:62 (2019).
Andriotis G, Elsayad K, Smart DE, Nalbach M, Davies DE, Thurner PJ. "Hydration and nanomechanical changes in collagen fibrils bearing advanced glycation end-products." Biomed. Opt. Exp.10(4):1841-1855 (2019).
Elsayad K, Kavirayani A. "Brillouin light scattering spectroscopy applications for liquid biopsies." Proc. SPIE, 104960W (2018).
Elsayad K, Palombo F. "Brillouin microspectroscopy: In search of the mechanical properties in biological systems well below a wavenumber." Spectroscopy Europe/Asia, 30(6):12-14 (2018).
https://www.spectroscopyeurope.com/system/files/pdf/SE_30-6_Brillouin.pdf
Garbrecht J, Hornegger H, Herbert S, Kaufmann T, Gotzmann J, Elsayad K, Slade D. "Simultaneous dual-channel imaging to quantify interdependent protein recruitment to laser-induced DNA damage sites." Nucleus 9(1):474 (2018).
DOI:10.1080/19491034.2018.1516485
Puhlyakova E., Aman AJ, Elsayad K, Technau U. "b-catenin dependent mechanotransduction dates back to the common ancestor of Cnidaria and Bilateria." Proc. Nat. Acad. Sci. USA 115(24):6231 (2018).
Elsayad Kc, and Heinze K. "Fluorescence Excitation, Decay, and Energy Transfer in the Vicinity of Thin Dielectric/Metal/Dielectric Layers near their Surface Plasmon Polariton Cutoff Frequency", in "Surface Plasmon Enhanced, Coupled and Controlled Fluorescence" (1st Edition, Edited by C. Geddes). 2017 John Wiley & Sons, Inc. [ISBN: 978-1-118-02793-6] (2017).
Schreiber B, Elsayad K, Heinze KG. "Axicon-based Bessel beams for flat-field illumination in total internal reflection fluorescence microscopy." Opt. Lett. 42(19):3880. (2017) (Editors Choice).
Slade D, Kaufmann T, Grishkovskaya I, Polyansky A, Kostrhon S, Kukolj E, Olek K, Sebastien H, Beltzung E, Mechtler K, Peterbauer T, Gotzmann J, Zhang L, Hartl M, Zagrovic B, Elsayad K, Djinovic-Carugo K. "A novel non-canonical PIP-box mediates PARG interaction with PCNA." Nucleic Acid Research 45(16):9741 (2017).
Elsayad Kc, Werner S, Gallemí M, Kong J, Sánchez ER, Zhang L, Jaillais Y, Greb T, Belkhadir Y. "Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging." Science Sig. 9(435):rs5 (2016) (Cover article).
Breuss M, Morandell J, Nimpf S, Gstrein T, Lauwers M, Hochstöger T, Braun A, Chan K, Sánchez Guajardo ER, Zhang L, Suplata M, Heinze KG, Elsayad K, Keays DA. "The expression of tubb2b undergoes a developmental transition in murine cortical neurons." Journ. of Comp. Neur. 523(15): 2161. (2015) (Cover article).
Heinze, KG, Elsayad, K. "Membrane Protein Localization by SpecON." Imaging & Microscopy 16:23 (2014).
https://analyticalscience.wiley.com/do/10.1002/imaging.4389
Elsayad Kc, Urich A, Tan, PS, Nemethova, M, Small, JV, Unterrainer, K, Heinze, KG, Spectrally coded optical nanosectioning (SpecON) with biocompatible metal–dielectric-coated substrates. Proc. Nat. Acad. Sci USA 110:20069 (2013).
Elsayad Kc, Urich, A, Nemethova, M, Small, JV, Unterrainer, K, Heinze, KG. "Fluorescence enhancements and spectral modifications near the cut-off frequency of plasmonic structures." Proc. SPIE 8234:82340W (2012).
Elsayad Kc, Suplata M, Heinze KG. "Using MATLAB to Achieve Nanoscale Optical Sectioning in the Vicinity of Metamaterial Substrates by Simulating Emitter-Substrate Interactions" in “Engineering Education and Research Using MATLAB" (Ed. A.H. Assi) [ISBN 978-953-307-656-0] (2011).
Elsayad Kc, Heinze, KG. "Temperature dependence of the near-field superlensing effect for single metal layers and stacked metal-dielectric films." Proc. SPIE. 7757:77573L (2010).
Elsayad Kc, Urich, A, Unterrainer, K, Heinze, KG. "Fast near-field imaging of spectrally broad sources using layered metallic structures." Proc. SPIE. 7757:77573M (2010).
Elsayad Kc, Heinze, KG. "Multifrequency parallelized near-field optical imaging with anistropic metal-dielectric stacks." Phys. Rev. A 81:053840 (2010).
DOI:10.1103/PhysRevA.81.053840
Neumüller, KG, Elsayad K, Reisecker, JM, Waxham, MN, Heinze, KG. "Photounbinding of calmodulin from a family of CaM binding peptides." PloS ONE 5:e14050 (2010).
DOI:10.1371/journal.pone.0014050
Elsayad Kc, Heinze, KG. "Defining a Superlens Operating Regime for Imaging Fluorescent Molecules." PloS ONE 4:e7963 (2009).
DOI:10.1371/journal.pone.0007963
Elsayad Kc, Carini, JP, Baxter, DV. "Measurement of linear-response Coulomb drag in insulating a-Si1-xNbx bilayer systems." Solid State Comm. 148:261 (2008).
Edited/Co-edited works:
Journal of Physics Photonics (by Institute of Physics). "Focus Issue on Brillouin Light Scattering for Biological and Medical Applications" (2024). Editors: Elsayad K, Kabakova I, Prevedel R, Zhang J.
https://iopscience.iop.org/collections/jpphoton-230608-255
Biomedical Optics Express. "Feature Issue on Brillouin Light Scattering Microspectroscopy for Biomedical Research and Applications" (Vol. 10, Iss. 5, 2019). Editors: Palombo F, Dehoux T, Fioretto D, Elsayad K (Lead Guest Editor).